Properties of enucleated cells. III. Changes in cytoplasmic architecture of enucleated BHK21 cells following trypsinization and replating.
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BHK21 cells enucleated with cytochalasin B were trypsinized and replated. Subsequent spreading and shape formation were monitored with light and transmission electron microscopy. Assembly and changes in distribution of microtubules, microfilaments and 100 Å filaments were related to these processes. These results indicate that cytoplasm contains the information necessary for the normal assembly and distribution of cytoplasmic fibers. © 1975.
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